The Yellamen had reached the FA Cup Preliminary round after overcoming Tadley Calleva at the second time of asking when they won the replay 4-2 after an initial 1-1 draw.

But manager Jeff Lamb could only field a patched up side as eight first-team players were missing for the trip to Bishops Cleeve through a combination of injuries and holidays.

Despite Lamb having to slot in at centre-back, Ascot held their own and the first-half was a cagey affair with not many chances at either end.

The Yellamen were looking to bounce back from their derby defeat at home to Bracknell Town last week, and would’ve been happy with their performance as they defended well and looked to break at every opportunity.

It was largely a frustrating afternoon though as attacking intent was often let down by decision making in the final third as they failed to test the opposing keeper enough times.

Chris Grace was forced into a superb save when Harlie Price went through one-on-one, and Ascot survived the scare as the scores remained level.

The visitors were also denied by a fantastic save themselves as they pushed on looking for the goal that would net them £1,925 in prize money and a place in the next round.

But Steve Benbow - the ex-Cheltenham Town goalkeeper - tipped Joe Grant’s flick over the bar to keep the scores level.

With the game heading towards full-time, it looked like a replay would be needed for the second round in a row for the Yellamen.

But a moment of magic in the 77th minute changed the game when Tustain found space on the edge of the Ascot box.

The substitute had only been on the pitch for seven minutes but he picked the ball up and lashed the ball past Grace into the bottom corner to give the hosts the lead.

Ascot threw caution to the wind looking to rescue the tie but they lacked the quality in the final third that would’ve hurt their opponents and had to settle for crashing out of the cup.

The Yellamen will look to bounce back to winning ways when they travel to Thame United on Saturday (3pm) before hosting Oxford City Nomads on Tuesday (7.45pm).